Tc1 and Tc0 clones show comparable levels of cytotoxicity.

Six Tc1 and Tc0/2 clones were stimulated with plate-bound anti-CD3 for 18 hours. Cells were washed, counted, and cocultured with OKT3 target cells at different effector to target cell ratios. After 4 hours, culture supernatants were collected and LDH levels were measured using a cytotoxicity detection kit; 1% Triton X was used to determine maximum lysis, and target cells in medium alone were used to determine spontaneous lysis. Spontaneous LDH release by effector cells was also determined. Color development was measured at 490 nm. Percentage lysis was calculated according to a modified standard formula: (OD experimental − OD spontaneous targets − OD spontaneous effectors)/(OD maximum − OD spontaneous targets) × 100.