Cytokine profiles of representative Tc1, Tc2, and Tc0 clones.

Clones were stimulated with plate-bound anti-CD3 and anti-CD28 MoAbs for 18 hours in the presence of Brefeldin A. Cells were washed, fixed, permeabilized, and incubated with anti–IFN-γ FITC and anti–IL-4 PE antibodies. Quadrants were set with reference to an unstimulated control. Stimulated cells stained with isotype control antibodies gave similar profiles. Percentages of cells positive for the particular cytokine are indicated.