Fig. 7.
Expression of HIF-1 proteins in A-172 cells.
Nuclear extracts (A) and whole-cell extracts (B) were prepared from A-172 cells under the following conditions: untreated or hypoxia-treated (12 hours), DMSO-treated (8 hours), or SNAP-treated (8 hours). The cells were subjected to Western blot analysis using mAb anti-HIF-1α.