EMSA with nuclear extracts from Hep3B cells.

Nuclear extracts (5 μg) from Hep3B cells, treated by 0.1% DMSO or 0.5 mmol/L SNAP (8 hours) or under normoxic or hypoxic conditions (12 hours), were incubated with WT HIF probe for 30 minutes at room temperature. SNAP-induced or hypoxia-induced complexes (H1 and H2) are indicated. D = DMSO, S = SNAP, N = normoxia, and H = hypoxia.