Effects of inhibitors on human VEGF promoter activity in A-172 cells.

The cells were exposed to the same conditions as Figure 1for 12 hours. (A) Effect of the NOS inhibitor (2 mmol/L L-NAME or 5 mmol/L AG). n = 8; no significant difference. (B) Effect of the GC inhibitor and/or protein kinase G activator (25 μmol/L MB, 1.25 μmol/L LY83 583, or 25 μmol/L ODQ and/or 800 μmol/L 8-Br-cGMP). n = 6; *P < 0.01 versus control in SNAP, #P < 0.05 and +P < 0.01 versus ODQ in SNAP, and 8-Br-cGMP in SNAP, respectively. (C): n = 6; *P < 0.01 versus control in hypoxic conditions. (C) Effect of SNP. *P < 0.01 versus control in hypoxic conditions.