Selectin binding and sLe^x expression before and during fucose therapy. Isolated neutrophils of a healthy control or of the LAD II patient before or during therapy (I through IV, as indicated) were analyzed by flow cytometry using the following reagents: (A and B) E-selectin–IgG (E-Sel-IgG) or P-selectin–IgG (P-Sel-IgG) in the presence of Ca²⁺ (bold, red line) or in the presence of EDTA (thin, green line), VE-cadherin–IgG (dashed, blue line); (C) P-selectin–IgG (bold, red line), preincubation with anti–PSGL-1 MoAb PL-1 before incubation with P-selectin–IgG (thin, orange line), VE-cadherin–IgG (dashed, blue line); (D) anti-sLe^x MoAb CSLEX-1 (bold, red line), irrelevant IgM control antibody (dashed, blue line). In each case, the fluorescence-labeled secondary antibody alone (negative control) was depicted in black (dotted line). Because the measurements of healthy control cells depicted in row IV were performed in parallel with the measurements depicted in row III, arrows in II/B and II/D mark the position of means of the healthy control signals as determined in parallel with these measurements.