Binding of LCA to fibroblasts. Fibroblasts from a healthy control and from the LAD II patient (as indicated) were grown in DMEM supplemented without (dashed, red line) or with 1 mmol/L (thin, green line) or 10 mmol/L (bold, blue line) L-fucose, respectively. The medium was changed daily and FACS analysis was performed after 5 to 7 days. Biotinylated LCA was detected with R-phycoerythrin–conjugated streptavidin. Binding of the second reagent alone is shown in black (dotted line). Binding of LCA to cells cultured in the absence of fucose could be completely blocked with -methyl-D-mannopyranosid, demonstrating that LCA binding to nontreated cells was fucose-independent (not shown).