Reduction of TAK-1–mediated cytotoxicity by treating target cells with a WT1 antisense oligonucleotide.

(A) WT1 protein expression in the leukemia cell lines MEG01 and TK91 treated with random (RA), WT1 sense (SE), or WT1 antisense (AS) oligonucleotides was examined by Western blot analysis (top). GAPDH protein expression was also examined by analyzing the same blot (bottom). (B) The cytotoxicity of TAK-1 to leukemia cell lines pretreated with random, WT1 sense, or WT1 antisense oligonucleotides for 72 hours was determined by 4-hour ⁵¹Cr release assays at an E:T ratio of 10:1.