Longitudinal TCR analysis of T cells responding to TT. Shown is the Southern blot of the TCRBV9 PCR products of TT-specific T-cell lines and clones obtained from UPN 285 at 3 consecutive years after allo-BMT using the TCR CDR3-specific TCRBV9S1 (PTGSG) oligonucleotide (Table 6) as hybridization probe. L1, TT-T cell line generated at 1 year after allo-BMT; C1, T-cell clone at 1 year post-BMT; L2/C2, line/clone 2 years post-BMT; L3, line 3 years post-BMT with TCRBV family (TCRBV1, TCRBV5, TCRBV8, and TCRBV9). In bold is the TCR CDR3 sequence for which the oligonucleotide probe was designed.