The effect of selective cell depletion in T-cell proliferation of CTX-SC.

SC and CTX-SC were obtained at day 6 (CD11b⁺ cell depletion) or day 10 (CD3⁺ cell depletion) after the last dose of PBS/CTX; 0.4 × 10⁶ cells/well were stimulated with Con A (1μg/mL), anti-CD3 (2μg/mL) plus IL-2 (50 U/mL), or IFN-γ (5 ng/mL) plus TNF-α (40 ng/mL) as indicated. The cells were assayed for (A) NO production after 72 hours of culture and (B) T-cell proliferation. Data are the mean ±SD of triplicate cultures.