Fig. 7.
iNOS expression in CTX-SC.
CTX-SC (0.4 × 106 cells/well) were cultured for 24-36 hours in the absence of or with Con A (1μg/mL) or anti-CD3 (2 μg/mL) plus IL-2 (50 units/mL). The cells were then recovered and stained with biotinylated anti-CD11b, anti-CD31, or PE-conjugated control TER-119 mAbs followed by AvPE, if required, for surface markers and FITC-conjugated antimouse iNOS. Isotypic controls showed <0.8% background in the selected regions. The results shown were obtained in 3 separate experiments.
