Expression of STAT5a and STAT5b in PHA blasts infected in vitro with 6 different HIV-1 viral strains. (Upper panel) Peak of RT values of kinetics of infection, observed up to 20 days, of PBMCs purified from a normal healthy donor and then infected in vitro with comparable moi of either 3 laboratory-adapted HIV-1 strains (IIIB/LAI, BaL, and 89.6) or 3 primary isolates (DU, SA, and BC). The peak of RT values was at day 13 for the IIIB, BaL, DU, and SA strains and at day 20 for the 89.6 and BC strains. (Lower panel) Immunoblotting of WCEs obtained at the indicated peaks of blast infection using first anti-STAT5a and then, after stripping of the filter, anti-STAT5b rabbit polyclonal Abs.