Patterns of reactivity of IgM of patients and healthy blood donors with self-antigens.

The densitometric profiles of reactivity of IgM with antigens in kidney (A,B) and RBC (C,D) extracts are depicted as individual profiles (black curves) in the case of 20 patients with WAIHA (A,C) and as individual profiles (black curves) in the case of 20 healthy donors (B,D). Gray areas show the densitometric pattern observed in the presence of the secondary anti-Fcμ antibody alone. Optical densities and migration distances are expressed as arbitrary units (AU). Migration distances of 200, 600, and 1000 AU correspond to apparent molecular weights of 200, 65, and 20 kDa, respectively.