Fig. 4. Western blotting of IgM with extracts of normal human kidney. / IgM in the plasma of patients with WAIHA and healthy blood donors was interacted at 20 μg/mL with a soluble extract of kidney antigens, as described in Materials and Methods. Lanes 1 to 10: IgM of patients; lanes 15 to 24: IgM of healthy blood donors; lanes 12 and 13: normal reference IgM (IVIgM); lanes 11 and 14: secondary anti-Fcμ antibody alone.
Fig. 4.

Western blotting of IgM with extracts of normal human kidney.

IgM in the plasma of patients with WAIHA and healthy blood donors was interacted at 20 μg/mL with a soluble extract of kidney antigens, as described in Materials and Methods. Lanes 1 to 10: IgM of patients; lanes 15 to 24: IgM of healthy blood donors; lanes 12 and 13: normal reference IgM (IVIgM); lanes 11 and 14: secondary anti-Fcμ antibody alone.

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