Time-lapse video microscopy of untransformed and BCR/ABL transformed cells in response to SDF-1. As described in Materials and Methods, Ba/F3 (A/B) and BCR/ABL-transformed Ba/F3 (C/D) cells were visualized by time-lapse video microscopy, without (A/C) and with (B/D) SDF-1. Ba/F3 cells have increased membrane ruffling, microspikes, and formation of pseudopods in response to SDF-1. In contrast, Ba/F3.p210^BCR/ABL cells had enhanced cell motility (as characterized by membrane ruffling, formation of pseudopods, formation of filopodia, and formation of uropod structures) with or without SDF-1.