American Society of Hematology

Fig. 1.

$Fig. 1. Cellular sources of IFN-γ secretion in response to autologous LCLs. / PBMCs from a healthy EBV-seropositive donor FT were sequentially fractionated into CD8+, CD4+, CD56+, and CD8−CD4−CD56−cell populations using CD8 and CD4 Dynal-beads and CD56 Microbeads. Each cell population was incubated with autologous LCL stimulators for the detection of IFN-γ secretion in an ELISPOT assay. Each bar represents the mean ± SD of triplicate wells. The numbers of spots/1 × 106 cells of the respective cell population are shown on the Y axis.$

Cellular sources of IFN-γ secretion in response to autologous LCLs.

PBMCs from a healthy EBV-seropositive donor FT were sequentially fractionated into CD8⁺, CD4⁺, CD56⁺, and CD8⁻CD4⁻CD56⁻cell populations using CD8 and CD4 Dynal-beads and CD56 Microbeads. Each cell population was incubated with autologous LCL stimulators for the detection of IFN-γ secretion in an ELISPOT assay. Each bar represents the mean ± SD of triplicate wells. The numbers of spots/1 × 10⁶ cells of the respective cell population are shown on the Y axis.

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