Effects of induced LMP-1 expression on CD99 molecules.

tTA-BJAB and LMP-1-BJAB cells were grown in the presence (+) or absence (−) of tetracycline for 2 days before being harvested for indirect immnunofluorescence staining for CD99 (A) and MHC class I (B) with the use of the DN16 and W6/32 monoclonal antibodies, respectively. Stained cells were analyzed by flow cytometry, and the relative antigen intensity was calculated.