Characterization of LMP-1 overexpressing IM9 cells.

(A) Slow growth rate of LMP-1 overexpressing cells. The same number (5 × 10⁴/mL) of cells were plated in 10% FBS-DMEM media at day 0. The total cell numbers were counted at the indicated days. LMP-1 overexpressing cells show slower kinetics of cell proliferation than do vector-transfected cells. (B) Cell cycle analysis. Flow cytometric analysis was performed on asynchronously proliferating vector-transfected or LMP-1–transfected IM-9 cells.