H-RS phenotype generated by LMP-1 overexpression in IM9 cells.

(A, B) Both Vec-TF (A) and LMP-1-TF (B) IM9 cells were morphologically examined with Wright’s and Giemsa staining. LMP-1 overexpressing IM9 cells showed typical H-RS morphology. (C, D) Vec-TF (C) IM9 cells and LMP-1-TF (D) IM9 cells were stained with anti–LMP-1 monoclonal antibody. LMP-1 expression was identified by incubation with FITC-conjugated goat anti-mouse antibody, and then was analyzed by confocal microscopy. Vec-TF cells showed minimal expression of LMP-1. (E, F) Vec-TF (E) IM9 cells and LMP-1-TF (F) IM9 cells were stained with FITC-conjugated anti-CD15 monoclonal antibody. While no CD15 expression was detectable in Vec-TF cells, the majority of enlarged LMP-1-TF cells showed relatively high expression of CD15 in Golgi and cytoplasmic regions. PI staining was included for nuclear staining in all samples except A and B. All slides were processed in parallel and photographed under identical magnification (original magnification ×630).