Fig. 7.
Effect of point mutations on the activity of the cyclin A1 promoter. (A) After introducing mutations into potential transcription factor binding sites of the cyclin A1 promoter (335-bp fragment), luciferase constructs were transfected into KCL 22 cells that express high levels of c-myb (Fig 3). Bars represent the mean and SEM of at least 3 independent experiments. (B) The wild-type cyclin A1 reporter construct or the myb 1 site mutation of the cyclin A1 promoter was cotransfected with either c-myb or empty vector into CV-1 cells. These experiments were performed using the Dual Luciferase Assay system and the pRL-SV40 vector for standardization.

Effect of point mutations on the activity of the cyclin A1 promoter. (A) After introducing mutations into potential transcription factor binding sites of the cyclin A1 promoter (335-bp fragment), luciferase constructs were transfected into KCL 22 cells that express high levels of c-myb (Fig 3). Bars represent the mean and SEM of at least 3 independent experiments. (B) The wild-type cyclin A1 reporter construct or the myb 1 site mutation of the cyclin A1 promoter was cotransfected with either c-myb or empty vector into CV-1 cells. These experiments were performed using the Dual Luciferase Assay system and the pRL-SV40 vector for standardization.

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