EMSA demonstrating binding of C/EBPɛ and C/EBP to the putative C/EBP site at position −55 of the lactoferrin promoter. Bars point to binding of either C/EBPɛ (lanes 2 through 7), or C/EBP complex (lanes 9 through 14) to double-stranded³²P γATP-labeled oligonucleotide– containing C/EBP site (–55) in lactoferrin promoter. Binding is specifically competed by cold oligonucleotides (lanes 3, 4 and 10, 11), but not by mutated oligonucleotides (lanes 5, 6 and 12, 13). The complexes did not bind to labeled mutated oligonucleotides (lanes 7 and 14). Both C/EBPɛ and C/EBP were supershifted by specific antisera (lanes 8 and 15, respectively).