FCA of RBCs and reticulocytes in a mouse mosaic forlox-▵Piga-lacZ (▵PIGA, mouse I.D. 3.1; Table 4) and a normal age matched control mouse. RBCs were stained with thiazole orange and PE-conjugated anti-CD24 antibodies. The left upper quadrant shows the proportion of mature normal RBCs (83%), the left lower quadrant corresponds to the mature PIGA(−) cells (11%), right upper quadrant normal reticulocytes (4%), and the right lower quadrant displays PIGA(−) reticulocytes (1%).