Fig. 3.
Effect of Tax expression on hiNOS mRNA induction. (A) JPX-9 cells were incubated with various concentrations of CdCl2 for 48 hours and then collected for preparation of whole-cell extracts. Whole-cell extracts isolated from JPX-9 cells were subjected to immunoblotting with anti-Tax antibody. (B) PCR analysis of reverse-transcribed hiNOS mRNA in JPX-9 and JPX-9/M cells cultured in medium alone (−) or medium containing 20 μmol/L CdCl2(+) was performed with the hiNOS oligonucleotide primer pair. Amplified products were electrophoresed.
