Annexin-V staining and TCR expression on Nef⁺ transduced thymocytes. Bivariate dot plots of flow cytometric measurement of Nef⁺ transduced thymocytes 30 days after injection of transduced CD34⁺ CB precursor cells. (A) Annexin-V versus EGFP expression. (B) TCR-β-PE versus EGFP expression. (C) TCR-β-PE and TCR-γδ-PE versus CD3-APCy expression of thymocytes also used for staining showed in (B), gated on EGFP⁺ cells. Quadrants were set to include 99% of cells stained with isotypic controls and EGFP⁻ cells in lower left quadrant. Figures indicate percentage of cells present in corresponding quadrants.