Intrathymic development of transduced CD34⁺CB progenitor cells in vivo. Bivariate dot plots of flow cytometric measurement of Nef⁻ transduced cells (top) and Nef⁺ transduced cells (bottom). (A) CD4-PE versus EGFP expression of SCID-hu thymocytes 30 days after injection of transduced CD34⁺ CB precursor cells. (B) CD8β-PE versus CD4-APCy and CD3-APCy versus CD27-PE expression of thymocytes shown in (A), gated on EGFP⁺ cells. Quadrants were set to include 99% of cells stained with isotypic controls and EGFP⁻ cells in lower left quadrant, except arbitrarily positioned to delimit DP cells in CD8β versus CD4 stainings. Figures indicate percentage of cells present in corresponding quadrants.