Effect of IL-10 on the turnover rate of CIS3 mRNA in LPS-stimulated PMN. PMN were cultured with 1 μg/mL LPS in the presence or absence of 100 U/mL IL-10. After 3.5 hours, actinomycin D (5 μg/mL) was added and the cells were further cultured for the indicated times. Total RNA was prepared and analyzed by Northern blotting with CIS3, TNF, and actin cDNA probes. This experiment is representative of 3.