Dose-dependent effect of IL-10 on CIS3 mRNA expression in neutrophils. PMN were incubated in the presence or absence of increasing concentrations of IL-10 for 2 hours before total RNA extraction. For comparative purposes, RNA was also extracted from PMN stimulated with GM-CSF and from autologous PBMC. CIS3 and IL-1ra gene expression was analyzed by Northern blot. This experiment is representative of 2.