Panel L is stained for the regulatory subunit (γ-GCS_l), which has a molecular weight of 28,000 (arrow). Panel H is stained for the catalytic subunit (γ-GCS_h), which has a molecular weight of 73,000 (arrow). The first lane is a molecular weight marker: 175,000, 83,000, 62,000, 47,500, 32,500, 25,000, 16,500, and 6,500, respectively, from the top. Lanes 2 and 3, hemolysate from a normal subject; lane 4, hemolysate from the patient; lanes 5 and 6, hemolysates from the mother and brother of the patient, respectively. A nonspecific band observed on the third lane of both sheets may represent an aggregation product formed during preparation of the samples.