Fig. 1.
Fig. 1. FACS analysis of anti-CD16 scFv binding to CD16+ granulocytes. The granulocytes were incubated with 1 μg of parental MoAb A9 or 1 μg purified anti-CD16 scFv. The scFv binding was detected with the anti–c-myc MoAb 9E10 and fluorescein-conjugated goat antimouse IgG. A CD16− cell line (SW 480) was used as a negative control.

FACS analysis of anti-CD16 scFv binding to CD16+ granulocytes. The granulocytes were incubated with 1 μg of parental MoAb A9 or 1 μg purified anti-CD16 scFv. The scFv binding was detected with the anti–c-myc MoAb 9E10 and fluorescein-conjugated goat antimouse IgG. A CD16− cell line (SW 480) was used as a negative control.

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