Fig. 4.
![Fig. 4. Effect of cytoplasmic domain deletion on receptor internalization. Cell surface glycoproteins were labeled with [3H]galactose, and receptor internalization was determined as described in Materials and Methods. The values shown are the average of 2 independent determinations. (□) The percentage of surface EPO-R internalized in 40 minutes at 37°C in the absence of EPO (−EPO); (▪) the percentage of surface EPO-R internalized in 40 minutes at 37°C in the presence of EPO (+EPO). Total cell surface glycoproteins internalization was determined from anti–EPO-R immunoprecipitates of lysates from (3H)galactose-labeled parental 32D cells that do not contain an EPO-R (total surface GP).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/94/8/10.1182_blood.v94.8.2667.420k27_2667_2675/7/blod42027004x.jpeg?Expires=1724247585&Signature=SB7tUw8-EozhL5nqJebRp2btscS11K~yLWISh0wKyqYUBWhmzUxMvjFEQamqdoC-PH7tb-7qqEx81MyQemUqZKxa1UjyQQIE0sEyZu7iEQwfmYBZjC3AEb4JvCxFieIqWejvVLAW~kBsNeSKB--rDnrO5DzENdn2iZlIJAzfqIYOLc4hmPacGZTPuNfnfW2pyCzgYqAIBQqBLXFgmI0L27dr985ryXRoyveze5nAxVarxthlF7~wfM3zEhEYVDkCX9xVqbrqZbivWHCh0Wf0tZxOrJ5bfPqhtlLjvgYiy1Lx~V7pxRPVED9l7nrY~gsHEw9~4sCWsdmB7f6sgAoDOA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of cytoplasmic domain deletion on receptor internalization. Cell surface glycoproteins were labeled with [3H]galactose, and receptor internalization was determined as described in Materials and Methods. The values shown are the average of 2 independent determinations. (□) The percentage of surface EPO-R internalized in 40 minutes at 37°C in the absence of EPO (−EPO); (▪) the percentage of surface EPO-R internalized in 40 minutes at 37°C in the presence of EPO (+EPO). Total cell surface glycoproteins internalization was determined from anti–EPO-R immunoprecipitates of lysates from (3H)galactose-labeled parental 32D cells that do not contain an EPO-R (total surface GP).
