FACS profiles of the blood cells 34 weeks after transplantation. The peripheral blood cells were stained in three colors for Ly5.1 (except for red blood cells), a lineage marker, and a GPI-anchored protein. Except for red blood cells, cells were first gated by Ly5.1 expression. Right panels show the surface expressions of GPI-anchored proteins in various lineages of cells from a mouse transplanted with mosaic fetal liver cells. Left panels show those from a mouse transplanted with liver cells of a normal littermate embryo. (A) Erythrocytes stained for TER119 and HSA. (B) Granulocytes stained for Mac-1 and HSA. (C) Monocytes stained for Mac-1 and HSA. Granulocytes and monocytes were distinguished according to light scattering profiles. More than 99% of granulocytes and monocytes were Ly5.1-positive, donor-derived cells. (D) B cells stained for B220 and HSA. More than 99% of B cells were of donor origin. (E) CD4 T cells stained for CD4 and Thy1. Ninety-five percent of CD4 T cells were of donor origin. (F) CD8 T cells stained for CD8 and Thy1. Ninety percent of CD8 T cells were of donor origin.