Fig. 5.
![Fig. 5. Autoradiogram showing phosphoproteins generated by protein kinase(s) present in immobilized erythrocyte immunoprecipitates. Sephacryl S-300 column fractions no. 40 through 51, combined and concentrated 6-fold, were incubated with agarose-linked antiphosphotyrosine (PT66) in the presence of low ionic strength buffers. Immobilized immunoprecipitates were incubated with protein kinase buffer augmented with 1 μmol/L [γ-32P]ATP (4 × 107 dpm) and 20 mmol/L magnesium acetate. The 12-, 30-, 36-, 42-, 60-, 72-, and 165-kD phosphoproteins are indicated on the right. Molecular mass markers (6.5 to 204 kD) are on the left.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/94/8/10.1182_blood.v94.8.2778.420k10_2778_2789/7/blod42010005w.jpeg?Expires=1723915789&Signature=st3o5zf-gZlDU9FLcDSLmKXKwwaNDNE8ho638GpAAOWCTWtvrEkvc4gxQwZ~Hlqkw2Z7MGqIigaR3-O11AvI9TgGuNcbVVK7xGsG4lt2Z0LlQ8~hKRewxxj-ke7svlkHUwR1SjrnGX9nBe9WaHeBQkGxchY5bLr-dinrRg2Ju6Bz4Zyv1VFE68ygilVM1pzo2HXMtFmbLEQ-Xi0fKy23IaHwc1p3EL9Phc6ZEtofda0UHsNqm8uR4s8A8UufnAuDU14BGK0d2c7vSx7kj2p1CRGsF3h4~gRyC8fLWXPI8sKhFOzNyqiMtD-tQR7K4MlEC2X87VpLuihaZZnGQH6QaA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Autoradiogram showing phosphoproteins generated by protein kinase(s) present in immobilized erythrocyte immunoprecipitates. Sephacryl S-300 column fractions no. 40 through 51, combined and concentrated 6-fold, were incubated with agarose-linked antiphosphotyrosine (PT66) in the presence of low ionic strength buffers. Immobilized immunoprecipitates were incubated with protein kinase buffer augmented with 1 μmol/L [γ-32P]ATP (4 × 107 dpm) and 20 mmol/L magnesium acetate. The 12-, 30-, 36-, 42-, 60-, 72-, and 165-kD phosphoproteins are indicated on the right. Molecular mass markers (6.5 to 204 kD) are on the left.
