Fig. 6.
![Fig. 6. Class I restriction of the IFNγ production in an EBV-specific CD8+ CTL line. EBV-specific CTLs were stimulated by autologous ([B] HLA-A24/A26, B52/B62, and C3) and various allogeneic LCLs ([C] HLA-A11/A26, B62, and C3/C4; [D] A11/A24, B61/B62, and C4; [E] A2/A24, B7/B61, and C7; [F] A2, B35/B46, and C1/C3). The underlined alleles were shared by CTLs and each LCL line. Unstimulated CTLs were also incubated, fixed, and then mixed with autologous LCLs before staining (A). The same CTLs were also stimulated by autologous LCLs in the presence of an anti-class I MoAb (G) or an isotype-matched MoAb (H). The frequency of CD8+/CD69high T cells that produced IFNγ is shown as a percentage of the total number of CD8 cells.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/94/9/10.1182_blood.v94.9.3094/4/blod42114006x.jpeg?Expires=1724242794&Signature=x9kensWAX86zR5db~ocfzXktTj3G8o1X2DqnhTm3ydylpAzZQN4i5xBaDRAKTK2Tn9mdMXdYthU2PzojTnLc0LCDwDygx0mwpBPHGCPGUnfo~iDcuzp3Kcaq3tq2RKbwgFcZCiuPRiW5UCR6b9gG0Ba3SEE3nDb3X8byityER1BmCS9BO46sZWPnZrV6A6gbxYDBXbICPTfNe~~IE9kvc8fCKLFyhr-0p3pKz6Xsv4X7MHUrS1YY6m1IcTlysWjl4PoKcUGUU-QlUeArKkEH4kBEBH7F0X81L3V1TXkPv6AKC8D8ST6vdGyfRzPalDdGSYgr6WFeR-gUKm31YPBcGw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Class I restriction of the IFNγ production in an EBV-specific CD8+ CTL line. EBV-specific CTLs were stimulated by autologous ([B] HLA-A24/A26, B52/B62, and C3) and various allogeneic LCLs ([C] HLA-A11/A26, B62, and C3/C4; [D] A11/A24, B61/B62, and C4; [E] A2/A24, B7/B61, and C7; [F] A2, B35/B46, and C1/C3). The underlined alleles were shared by CTLs and each LCL line. Unstimulated CTLs were also incubated, fixed, and then mixed with autologous LCLs before staining (A). The same CTLs were also stimulated by autologous LCLs in the presence of an anti-class I MoAb (G) or an isotype-matched MoAb (H). The frequency of CD8+/CD69high T cells that produced IFNγ is shown as a percentage of the total number of CD8 cells.
