FACS sorting regions used for preparation of c-kit⁺ Sca-1⁺ CD34⁻ and c-kit⁺ Sca-1⁺ CD34⁺ cell populations. Lin⁺ cells had been removed from mononuclear marrow cells by using immunomagnetic beads before staining for FACS sorting. The Lin⁻ cells were enriched for c-kit⁺ Sca-1⁺ cells using the R1 and R4 windows and again sorted on the basis of CD34 expression using R2, R3, R5, and R6 windows.