Fig. 1.
![Fig. 1. Identification and purification of the Btk-SH2 domain-binding protein. (A) Lysates of RAMOS cells stimulated with anti-μ-antibody (+) or without stimulation (−) were incubated with the fusion protein of GST and the wild-type Btk-SH2 [SH2(Wild)] or mutated Btk-SH2 [SH2(R307K)] domain. Binding phosphoproteins were detected by immunoblotting with the antiphosphotyrosine (anti-pTyr) antibody 4G10. (B) Ponceau S staining of the purified proteins on PVDF membrane. (C) Peptide mass map obtained by MALDI mass analysis. The result obtained from the peptide mixture generated byAchromobacter protease I digestion of the 68-kD protein is representatively shown.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/94/7/10.1182_blood.v94.7.2357.419k40_2357_2364/6/blod41940001w.jpeg?Expires=1725025981&Signature=DAs~knHIy~cXyM-gdrPFlQ9Nddj20ETkPmbkLjymlOutQsgjlQ43H624EIN09T3z~8MVA~ndZllj9kMWSS3vQM29Wb7L2WpTFCG96ysp~oN9RdxR3GBOWhRYRvDou9ZFxy2Wwo8ZpIYNtODLonHqdiHtYGGJg-7LqlaANqashngap~Wk6jfnclx3FPjBZKmHZzYjY7fFJONkyNGPTXPUKD8WUF3jLCrbbT~FXOOOt6hhbxTqHKGiHIM9kPzkXbP8sQZIciOT5ZqEF~a8Wo2PV~~ZSR-vGulwBDrVEoQC2rPsS05kWpq7bbRG~zpBaMfDjEpz8GaEBIq29HaZku7GvA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Identification and purification of the Btk-SH2 domain-binding protein. (A) Lysates of RAMOS cells stimulated with anti-μ-antibody (+) or without stimulation (−) were incubated with the fusion protein of GST and the wild-type Btk-SH2 [SH2(Wild)] or mutated Btk-SH2 [SH2(R307K)] domain. Binding phosphoproteins were detected by immunoblotting with the antiphosphotyrosine (anti-pTyr) antibody 4G10. (B) Ponceau S staining of the purified proteins on PVDF membrane. (C) Peptide mass map obtained by MALDI mass analysis. The result obtained from the peptide mixture generated byAchromobacter protease I digestion of the 68-kD protein is representatively shown.
