Semiquantitative duplex RT-PCR analysis of PTPROt in primary DLB-CLs, DLB-CL cell lines and normal splenic B cells. Five primary DLB-CLs, 4 DLB-CL cell lines (DHL-4, DHL-7, DHL-8, and DHL-10), and normal unsorted splenic B cells from 2 donors were analyzed for PTPROt expression by semiquantitative duplex RT-PCR. The abundance of PTPROt in a given sample was determined by comparing the intensity of coamplified PTPROt and ABL PCR products.