Colocalization of GH with vWF. GHrAd-infected HUVEC were fixed, permeabilized, and stained with rabbit anti-GH antibody followed by rhodamine-conjugated sheep antirabbit antibody and then FITC-conjugated sheep anti-vWF antibody. Images were obtained by scanning confocal microscopy at 0.36-μm intervals in the Z-axis using a 100× objective. Shown is a representative scan from the approximate midsection of the Z-axis. The red and green images were merged to demonstrate colocalization, which appears yellow.