Fig. 3.
Fig. 3. Other CD34+/CD38−cells initially gave rise to 2 daughter cells that appeared equivalent, but then divided asymmetrically after the second or third division. This figure depicts a typical example, ie, 1 parent cell gave rise to 2 daughter cells (at 38 hours), which in turn gave rise to 4 cells, with 1 of them then remaining quiescent and the other 3 multiplying symmetrically in subsequent divisions, yielding 7 cells at 60 hours, ie, on day 3. Thereafter, 1 cell maintained bright PKH26 fluorescence, whereas the other 6 multiplied to yield hundreds of cells after 9 days, which all showed very dim to nondetectable fluorescence.

Other CD34+/CD38cells initially gave rise to 2 daughter cells that appeared equivalent, but then divided asymmetrically after the second or third division. This figure depicts a typical example, ie, 1 parent cell gave rise to 2 daughter cells (at 38 hours), which in turn gave rise to 4 cells, with 1 of them then remaining quiescent and the other 3 multiplying symmetrically in subsequent divisions, yielding 7 cells at 60 hours, ie, on day 3. Thereafter, 1 cell maintained bright PKH26 fluorescence, whereas the other 6 multiplied to yield hundreds of cells after 9 days, which all showed very dim to nondetectable fluorescence.

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