American Society of Hematology

Fig. 6.

$Fig. 6. (A) May-Grünwald-Giemsa–stained CD34+97A6+ BM cells derived from MACS-selected CD34+ cells after staining with anti–CD34-FITC and 97A6-PE, and fractionation by FACS sorting; the cells in a-d show heterogeneity. (B) Colony from CD34+97A6+ BM cells after day 16 of culture in semisolid methylcellulose-containing medium. (C) Mast cells from CD34+97A6+ BM cells after 35 days of culture in serum-free medium in the presence of 100 ng/mL SCF. (D) May-Grünwald-Giemsa–stained cells derived from a bipotent basophil/eosinophil colony type shown in (B). Figure 6D (a, b) and (c, d) show representative basophils (basophilic granules) and eosinophils (eosinophilic granules) of different developmental stages from this colony.$

(A) May-Grünwald-Giemsa–stained CD34⁺97A6⁺ BM cells derived from MACS-selected CD34⁺ cells after staining with anti–CD34-FITC and 97A6-PE, and fractionation by FACS sorting; the cells in a-d show heterogeneity. (B) Colony from CD34⁺97A6⁺ BM cells after day 16 of culture in semisolid methylcellulose-containing medium. (C) Mast cells from CD34⁺97A6⁺ BM cells after 35 days of culture in serum-free medium in the presence of 100 ng/mL SCF. (D) May-Grünwald-Giemsa–stained cells derived from a bipotent basophil/eosinophil colony type shown in (B). Figure 6D (a, b) and (c, d) show representative basophils (basophilic granules) and eosinophils (eosinophilic granules) of different developmental stages from this colony.

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