Fig. 7.
Effects of AS2 on the production of pRb. Cells at an initial concentration of 5,000/mL were incubated in medium supplemented with FL at 50 ng/mL and IL-3 at 25 ng/mL to promote monocytic differentiation. Oligo buffer, sense-orientated oligonucleotides (SO2), scrambled oligonucleotides (SCR), or antisense RB oligonucleotides (AS2) were added on days 0, 5, and 10, each time at a concentration of 5.0 μmol/L. On days 3 and 5, 1.5 × 106 cells from each incubation were subjected to protein extraction, followed by specific immunoprecipitation with an anti-pRb antibody (sc-102; Santa Cruz), 6% SDS-PAGE, and Western blot analysis. pRb of different molecular weights are indicated with arrows (ppRb, hyperphosphorylated; pRb, hypophosphorylated).
