Frequency of TCR Vβ and V family in FHL patients. With densitometry, a semiquantitative assessment of V gene usage was made from the amount of hybridized products. The dotted line shows 15% of β-T cells. TCR V gene segments were regarded as highly expressed when their relative frequency was more than 15%. A highly expressed Vβ family member was observed in 3 of the 5 patients: Vβ5a and 5b of patient no. 2 and Vβ6 of both patients no. 4 and 5. A high frequency of a V family member was detected in all 5 patients: V3 and 9 in patient no. 2, V3 in patient no. 3, and V9 in patients no. 4 and 5.