Microsatellite alterations in adult acute leukemias. PCR amplification of the indicated microsatellites was performed as described in Materials and Methods on genomic DNA of AML patients at diagnosis (L) or relapse (Rel), and the resulting banding patterns were compared with those obtained at remission (R). Tumor-specific alterations consisted either of gain of new-length alleles (MSI), indicated by arrows, or loss of bands detected in constitutional DNA (LOH).