Calreticulin and calreticulin fragments inhibit tumor growth. Burkitt lymphoma cells (CA46 cell line, 1 × 107cells) were inoculated sc into BALB/c athymic mice, 6 weeks of age. (A) Beginning on the day of cell inoculation and continuing thereafter daily, 6 days/week, 12 mice were inoculated with control GST protein (▪, 20 μg/d × 14 days), and 13 mice were inoculated with GST-calreticulin (▵, 60 μg/d × 14 days). (B) Beginning on the day of tumor appearance, 5 mice were inoculated with control MBP protein (▪, 12.5 μg/d), 4 mice were inoculated with MBP-calreticulin (▵, 12.5 μg/d), and 4 mice were inoculated with MBP-vasostatin (○, 12.5 μg/d). (C) Beginning on the day of cell inoculation and continuing thereafter daily, 6 d/wk, 8 mice were inoculated with control MBP (▪, 20 μg/d × 14 days), 9 mice were inoculated with MBP-vasostatin (○, 30 μg/d × 14 days), and 9 mice were inoculated with ▵120 calreticulin (▵, 30 μg/d × 14 days).
