Fig. 7.
Comparative analysis of endothelial cell growth inhibition by MBP calreticulin (□), MBP–▵120-calreticulin (○), MBP-vasostatin (◊), MBP 120-180 calreticulin (▵) fragment, and MBP (⊞). Fetal bovine heart endothelial cells (800 cells/well) were cultured for 5 days in medium alone or medium supplemented with bFGF (15 ng/mL). Recombinant purified fusion proteins were added to culture at 0.4 to 32 nmol/L concentrations to bFGF-supplemented cultures. Proliferation was measured by 3H thymidine incorporation during the final 20 to 23 hours of culture. The results reflect the mean of triplicate cultures; SDs within 15% of the mean. The mean response of endothelial cells was 2,217 cpm when cultured in medium alone, and 23,377 cpm when cultured with bFGF alone.