Dose-dependency of inhibition of endothelial cell proliferation by calreticulin. Fetal bovine heart endothelial cells (800 cells/well) were cultured for 5 days in medium alone or medium supplemented with bFGF (25 ng/mL). Recombinant purified GST-calreticulin (1 μg/mL), control recombinant GST (0.03 to 1 μg/mL), calreticulin cleaved and purified from MBP-calreticulin (0.03 to 2 μg/mL), and MBP cleaved and purified from MBP-calreticulin (0.5 μg/mL) were added to endothelial cell cultures with bFGF (25 ng/mL). Proliferation was measured by 3H thymidine incorporation during the final 20 to 23 hours of culture; the results reflect mean cpm/culture. (A) Reflects the mean of 9 experiments, each performed in triplicate. (B) Reflects the mean of 2 experiments, each performed in triplicate.
