In vitro transduction efficiencies. (A) Semi-quantitative PCR for the β-gal and actin sequences was performed at the end of the ex vivo culture period. The β-gal is the vector sequence and the actin is the control sequence. (B) The graph summarizes the in vitro transduction efficiencies shown as a mean copy number per cell, calculated from the PCR results. The MOI used was different among monkeys. 94E068 had 2,000, RQ1307 and RQ1303 had 20,000, and RQ864 had 200,000 AAV genomic copies per lymphocyte. (□), AAV; (▪), retrovirus.