Fig. 6.
Fig. 6. Southern blot analysis of the 5′ region of the EphA3 gene in normal human peripheral blood and tumor cell lines. Samples shown in the top panel all express the EphA3 gene and samples shown in the bottom panel are EphA3 negative. LK63 and Nalm-1 are pre-B cell lines and Raji is a B-cell line. Jurkatt, HSB-2, and HPB-ALL are T-cell lines. Genomic DNA (10 μg) from each sample was digested withSacI (S), or double digested with SacI and AvaI (SA), SacI and PvuII (SP), and SacI andSacII (SS). AvaI and SacII are methyl-sensitive restriction enzymes. Digests were incubated overnight at 37°C, run on a 1% agarose gel, and transferred to a Zetaprobe membrane. The membrane was hybridized with the 1.4-kb SacI genomic fragment containing the EphA3 promoter.

Southern blot analysis of the 5′ region of the EphA3 gene in normal human peripheral blood and tumor cell lines. Samples shown in the top panel all express the EphA3 gene and samples shown in the bottom panel are EphA3 negative. LK63 and Nalm-1 are pre-B cell lines and Raji is a B-cell line. Jurkatt, HSB-2, and HPB-ALL are T-cell lines. Genomic DNA (10 μg) from each sample was digested withSacI (S), or double digested with SacI and AvaI (SA), SacI and PvuII (SP), and SacI andSacII (SS). AvaI and SacII are methyl-sensitive restriction enzymes. Digests were incubated overnight at 37°C, run on a 1% agarose gel, and transferred to a Zetaprobe membrane. The membrane was hybridized with the 1.4-kb SacI genomic fragment containing the EphA3 promoter.

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