Fig. 2.
![Fig. 2. Stage-specific suppression of CFU-Meg by TGF-β1. CFU-Meg colony assay was performed with CD34+ cells incubated with TGF-β1 (1.0 ng/mL) in a delayed addition fashion at days 0, 2, 4, 6, 8, 10, 12, and 14 ([▩] presence of TPO in culture medium; [▧] presence of TGF-β1 in the culture medium). CFU-Meg colony assays, without TGF-β1 and with TGF-β1 preincubation for 24 hours, were also performed.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/94/6/10.1182_blood.v94.6.1961/5/blod41817002x.jpeg?Expires=1722710537&Signature=AAqIU2oa7PiOhX89OX2vhhF6-3CnCOOvUu24AH0hd5eb1CLH11sDj~Xjk0LAVsOsz8RSQVbexN1yUmDejvfW~Ev~n2AKRGf4nmtocFsr1eJgH0ZNeTG2aUPF2B0qMp5GslGOcjqk8Cd-Nr9Mp3QMz1rDCR9BhnYzbR~XMSssPx9~2KS1p66bqPDOjc7wpsrNNOGLyeWEZEC1yZbEygm86ZTuHSiJqSfVfXXa079rFZjZH04d76KSr~u3SPYiy9jEmyPh-F58Ibm7w6Y75KVB7Ne-p8LM5v7hGIzOrBqU~45c-QFQ4ZHnn1gsrF2ko-yRT1wnJA-estt0k0vEhnakzw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Stage-specific suppression of CFU-Meg by TGF-β1. CFU-Meg colony assay was performed with CD34+ cells incubated with TGF-β1 (1.0 ng/mL) in a delayed addition fashion at days 0, 2, 4, 6, 8, 10, 12, and 14 ([▩] presence of TPO in culture medium; [▧] presence of TGF-β1 in the culture medium). CFU-Meg colony assays, without TGF-β1 and with TGF-β1 preincubation for 24 hours, were also performed.
