(A) Dose response of intracellular oxidation by SA. Eosinophils were incubated with medium control or the indicated concentration of SA (μmol/L). The mean green fluorescence representing the intracellular release of H₂O₂as determined by augmented R123 fluorescence (FL 1-H) is indicated. (B) Intracellular H₂O₂ increase (A) was associated with increased percentages of apoptotic nuclei. Eosinophils were incubated for 19 hours with medium control or the indicated concentration of SA and the hypodiploid DNA peak was measured using the PI method. Nuclei were flow cytometrically analyzed and data plotted on log histograms as red fluorescence intensity (x-axis) versus relative cell number (y-axis). Percentages of hypodiploid DNA peaks corresponding to apoptotic nuclei are indicated.