Fig. 4.
Fig. 4. Detection of HVMNE in the cell lines J94356PBMC and J94356SKIN by electron microscopy and DNA PCR. (A) EM of cell line J94356PBMCharvested 48 hours after induction with Na-butyrate (1 μmol/L). Extensive EM search in the same cell line without Na-butyrate induction failed to show viral particles (not shown). (B) Southern blot of PCR products using probe 536. Detection of a 536-bp DNA fragment in the J94356PBMC and J94356SKIN cell lines at 46 days in culture and after 226 days in culture in line J94356SKINby PCR using the generic primer pair DFASA/GDTD1B (described by Rose et al33).

Detection of HVMNE in the cell lines J94356PBMC and J94356SKIN by electron microscopy and DNA PCR. (A) EM of cell line J94356PBMCharvested 48 hours after induction with Na-butyrate (1 μmol/L). Extensive EM search in the same cell line without Na-butyrate induction failed to show viral particles (not shown). (B) Southern blot of PCR products using probe 536. Detection of a 536-bp DNA fragment in the J94356PBMC and J94356SKIN cell lines at 46 days in culture and after 226 days in culture in line J94356SKINby PCR using the generic primer pair DFASA/GDTD1B (described by Rose et al33).

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